Gene & Protein Expression Profiling

Zymetrix has expertise in characterizing the phenotypic state of cellular biomaterials, particularly in terms of their response to mechanical loads.  The core techniques that Zymetrix has available include the following:

  • Protein, DNA and RNA extraction and purification
  • Gel electrophoresis (SDS-PAGE), Western and functional expression analysis of conditioned media
  • Qualitative and quantitative gene expression profiling using PCR and RT-qPCR.  This includes an Applied Biosystems Viia7 real-time PCR with TaqMan®  Array Card block for medium to high throughput.
  • Measurement of gene expression, protein expression and drug penetration via imaging (confocal, microCT, MRI)